Serum Edos and you will Ad was indeed mentioned having fun with Estradiol EIA Package (Cayman Chemicals, MI) and you can androstenedione chemical immunoassay package (Oxford Biomedical Search Inc., MI), respectively. ELISAs away from T and you will E1 was in fact performed depending on the same means of 17,20?-P (Matsuyama et al., 1998b) and you will explained below briefly.
Stock solutions: Coating buffer, 0.05 M carbonate buffer pH 8.cuatro, containing 0.05% NaNstep 3; washing solution, 0.85% NaCl; blocking solution, 0.05 M PBS containing 0.1% BSA, 3% sucrose, and 0.005% thimerosal; assay buffer, 0.05 M borate buffer, pH 7.8, containing 0.1% BSA and 0.01% thimerosal; substrate solution, 0.2 M citrate buffer, pH 4.5, containing 0.01% H2O2, added 0.5% o-phenylenediamine immediately before use; stopping solution, 6 N H2SO4. All the solutions except for the stopping solution were stored at 4°C.
Next Antibody-coated Microtiter Dishes: Microtiter dishes (MS-3596 F/H dish, Sumitomo Bakelite Co.) was indeed decorated which have a hundred ?l for each really out-of goat anti-bunny IgG (15 ?g/ml in level barrier). Read More
